老平农发帖认领翻译的论文：综合水果酵素的医疗应用研究

牵牛花

            翻译是我老公，一定不是最好的翻译，求包涵。论文是2005年，有些内容会和现在的理论不一致，数据更是不得而知了，参考吧。



Faculty of Pharmacy
Multi Fruit liquid Fermented 2 yearsreports,
for summery To present as a topic ofresearch on wound healing
Present to
Dr.Chaiwat Chaiyasut
by
Miss Rosukon Poompanvong
Year, 2005
Multi – Fruit Enzyme For Healing Wounded,Internal and External used
Definition
Type Fruit
Gram
Honey
Water/cc.
zalaceca   
100
30

1,370

Longan  
100
30

1,370

Star  Fruit
100
30

1,370

Guava  
100
30

1,370

Tamarind   
100
30

1,370

Rambutan  
100
30

1,370

Longon  
100
30

1,370

Mangos  teen
100
30

1,370

Banana  
100
30

1,370

Sherry  
100
30

1,370

Papaya  
100
30

1,370

Pine  Apple
100
30

1,370

Jack  Fruit
100
30

1,370

Castard  Fruit  
100
30
1,370
1)    Airthight – Plastic Bottle * 1 Year * and the 2nd Change  to new plastic Bottle added 10 %  honey  (dehydration 80 %)

2)    Because, the Plastic should not be used longer than one year.
**The2 nd year** Check the quality on  pH,Conductivity.
Synonyms
SelectedVernacular names
Description
Plantmaterial of interest ; Fresh, Peel  (Generalappearance)
Organoleptic properties  
Mild
Aromatic
OdourNative Fresh smell
Initiallysweet, Sour, taste (100%)
MicroscopicCharacteristics.
     A description of the microscopicCharacteristics of Cross-Section of the aerial parts of the Fruit is Currentlyunavailable.
Geographicaldistribution
100 % Fruit is native in SouthEast Asia, Asia Mostly – in India, South China, Afarica, and West Indian. (???????????)
Generalidentity tests.
     Macroscopic examination and Thin-layerChromatography and high performance liquid Chromatography of the lipophilicConstituents and chicoric a in methanol extracts.
PurityTests, Microbiology
- TheTest for Salmonella spp. In Multi-Fruit Enzyme.
-TheMaximum acceptable limits of other micro – organisms are as follows
- Forpreparation of decoction : aerobic bacteria not more than 107  /g ; Escherichia coli-not more than 102/g,fungi – not more than 105/g
-Preparations for internal use : aerobic bacteria not more than 105/gor ml;
-fungi– not more than 104/g or ml;
-enterobacteria and certain Gram-negative bacteria not more than 103/gor ml;  fungi –not more than 102/gor ml;
-Escherichiacoli-o/g or ml.
-Preparationfor external use;
-aerobicbacteria not more than 102/g or ml;
Pesticideresidues
To be established in accordance withnational requirements. Normally, The maximum residue limit of aldrin anddieldrin in Fruit is not more than 0.05 mg/kg
for other pesticides, WHO guidelines onQuality control methods for medicinal
plants(4) and guidelines for predicting dietary intake of pesticide residues (5)
Heavy  metals.
Recommended lead and cadmium levels  are no more than 10 and 0.3 mg/kg,respectively, in the final dosage form of the plant material

Radio-activeresidues
For analysis of strontium-90, iodine 131, caesium -134, caesium -137, and plutonium -239, see WHOguidelines on quality control methods for medicinal plants
Otherpurity tests
Chemical tests and tests for acid –insoluble  ash dilute ethanal – solubleextractive, foreign organic matter, moisture, Total ash, and water – solubleextractive to be established in accordance with national requirements.
Chemicalassays.
1.  For essential oil (0.08-0.32 %) ; Chicoric a (1.2-3.1%) Quantitative analysis of             Multi-Fruit Enzyme, Chicoric a, isobutylamides, and other Constituents by high
2.  performance liquid chromatography
3.  Quantitative analysis of alkamides and caffeic a derivatives byThin – layer chromatography and high performance liquid chromatography.
MajorChemical Constituents
     A number of chemical entities have beenidentified, including alkamides, polyalkenes, polyalkynes, caffeic a derivatives,and polysaccharides
     The Volatile oil contains, among otherCompounds, borneol, bornyl acetate, pentadeca-8-(2-en-2-one, germacrene D, caryophyllene,and caryophyllene epoxide.
     Isobytylamides of C11-C10straight-Chain fatty acids with  olefinic or acetylenic bonds (or both) are found in the multi fruit liquid,  with the isomeric dodeca  (2E, 4E, 82, 10E/z) – tetraenoic  acid  isobutylamindes
     The caffeic acid ester derivative chicoricacid is the major active compound of this class found in the MultiFruit liquid,with a concentration range of 1.2-3.1 %
     Chicoric acid methyl ester and otherderivatives are also present.
     Polysaccharide constituents from MultiFruit liquid are two types : a heteroxylan of average relative molecular massabout 3,500 (e.g. PS-I), and anarabinorhamnogalactan of average relative molecular mass about 45,000(e.g.PS-II)
     Other Constituents include trace amounts ofpyrolizidine alkaloids (tussilagine 0.006 %) and isotussilagine).
     At these concentrations, the alkaloids areConsidered to be non toxic.
     Furthermore, because these alkaloids lackthe 1,2 – unsaturated necine ring of alkaloids such as senecionine  (Structure in Box) they are considered to benon – hepatotoxic, Structure of representative Constituents are presented below.
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Dosageforms.
MultiFruit liquid : preparations there of for internals and external use.
Medicinaluses. Used Supported by Clinical data.
     Multi Fruit liquid is administered orallyin supportive therapy for colds and infections of the respiratory and urinarytract.
     Beneficial effect is the treatment of theseinfections are generally thought to be brought about by stimulation of theimmune response. External uses include promotion of wound healing and treatmentof inflammatory skin conditions

     Uses described in folk medicine, notsupported by experimental or chemical data.
     Other medical uses claimed for Multi Fruitliquid include treatment of yeast infections, side – effects of radiationTherapy, rheumatoid arthritis, blood poisoning, and food poisoning.
Pharmacology.
Experimentalpharmacology
     Current Claims of the effectiveness ofMulti Fruit liquid as a stimulator of the immune system are based on numerousscientific studies. The immuno stimulant effect is brought about by threemechanisms : activation of phagocytosis and stimulation of fibroblasts ;increasing respiratory activity ; and increased mobility of the leukocytes. Phagocyticactivity of standardized extract of the MutiFruit liquid has been determined.
A lyophylisate of Multi Fruit Liquid significantlyincreased the percentage of phagocytizing human gramulocytes and stimulated thephagocytossis of yeast particles in vitro. Inhibition of the activity of theadrenal cortex, stimulation of the production of properdin (a serum proteinwhich can neutralize bacteria and viruses), and stimulation of interferonproduction have also been reported after multiFruit liquid treatment.
     The pharmacological activity of Multi Fruitliquid has been attribute to five component fraction in addition to theessential oil, namely the alkylamidles, caffeic a  derivatives, polyalkynes, polyalkenes, andpolysaccharides.
     The lipophilic amides, alkamides, andcaffeic a derivatives uppear to contribute to the immunostimulant activity ofthe alcoholic MultiFruit liquid extracts by stimulating phagocytosis ofpolymorphonuclear neutrophil granulocytes.
     High molecular weight polysaccharides,including heteroxylan, which activates phacgocytosis, and arabinogalactan,which promotes the release of  tumournecrosis factor and the production of interleukin -1 and interferon beta, havealso been implicated in the activity of the Multi Fruit liquid when takenorally. The overall immuno stimulant activity of the alcoholic and appears todepend on the combined effects of several constituents.
     Topical applications of MultiFurit  liquid have been traditionally used to promotewound healing. The first published work on the mechanism of this action was byHealth Farm, Rayong, Thailand. Dr. Rosukon Poompanvong,who investigated the effect of MultiFruit liquid on strepto cocal and tissuehyaluronidase. Inhibition of tissue and bacterial hyaluronidase is thaught tolocalize the infeetion and prevent the spread of causative agents to otherparts of the body. In addition to the direct antihyaluronidase activity, anindirect effect on the hyaluronic acid-hyaluronidase system has been reported.
Stimulation of new tissue production byincreasing fibroblast activity, and stimulation of both blood and tissueproduced phagocytosis, appear to be involed in this mechanism.                    
The polysaccharide fraction appears topromote wound healing by forming a hyaluronic acid – polysaccharide complexthat indirectly leads to the inhibition of hyaluronidase.
     In invitro experiments, an ethanol extract (65%) by volume of Multi Fruitliquid fermented (2 Yrs.) inhibited the Concentration of collagen by mousefibroblasts, measured by the collagen lattice diameter.
     Mouse macrophages pretreated withpolysaccharides that were isolated from the supermatant of Multi Fruit liquidFermented (2 yrs.) increased production of tumour necrosis fact of alpha,interleukin-1, and interferon beta -2 and increased cytotoxicity against tumourcells and micro-organisms (Leishmania enreitii)     
     Purified palysaccharides isolated  Multi Fruit liquid fermented (2 yrs.) enhancedthe spontaneous  motility of humanpolymorphonuclear leukocytes under soft agar and increased the ability of thesecells to kill Staphylococcusaureus.
     Human monocytes were activated to secretetumour necrosis factor alpha, interleukin 1, and interleukin -6 while theexpression of class 11 human leukocyte antigens was unaffected.
Forpurified caffeic acid derivatives, antiviral activities have been demonstrated.
     Incubation of vesicular stomatitis virus(VSV) with 125 μg/ml of chicoric acid for 4 hours reduced the number of viralparticles in mouse L-929 murine cells by more than 50 %
Clinicalpharmacology
     Recently 6 controlled clinical trials weresystematically reviewed at health farm Trials studied the prophylaxis orcurative treatment of infections, four trial studied the reduction of sideeffects of chemotherapy, and three investigated the modulation of specificimmune parameters.
     The reviews concluded that MultiFruitliquid fermented (2 Yrs.) are efficacious immunomodulators.
     However, it also concluded that there wasin sufficient evidence for clear therapeutic recommendations as to whichpreparation or dosage to use for a specific indication.
     A large – scale longitudinal trial (500  patients) studied the effects of anointment Containing a lyophylisate. The ointment was used to treat inflammatoryskin conditions, wounds,czema, burns, herpes simplex, and varicose ulcerationsof the legs.

     The Therapeutic benefit from the ointmentwas observed in 85.5 % of the cases. The treatment periods ranged from  7  to 15 days.

Contraindications
    External use – Beaware when open thebottle of Muti Fruit liquid fermented for 2 yrs. There is explosion andcould  hit the body, So ! has to removethe cover in the horizontal layer.

Harmlessto any allergic.
Internaluse.
     should not be used in the seriousconditions such as of AIDS, HIV infection, collagenosis multi – sclerosis, andautoimmune disorder.
Warning
Noinformation available.
PrecautionsGeneral
     Has to use it with continuiously about 7weeks and once a week until the symptom has recovered.
Carcinogenesis,mutagenesis, impairment of fertility.
     Mutagenicity and carcino genicity testresults were negative. Doses up to a polysaccharide Concentration of 500 mg/mlcaused no incrase in sister chromatid exchange or structural chromosomeaberrations.
Pregnancy: teratogenic effects
     There are no reliable studies on thissubject. Therefore, administration of the drug during pregnancy is notrecommended.
Paediatricuse
Oral administration of Multi Fruit liquidfermented (2years) is recommended for
justborn, by drop direct, when they are any small children and on the advice ofphysician and be used for external treatment.
Otherprecaution
     No information available concerning drug interactions,drug and laboratory test interactions, or non – teratogenic effects onpregnancy.
Adversereactions.
     Occasionally, has some inflammatory becauseof the healing period and must to continuing until the symptom gradullarybetter and healed, not longer than 2-3 days after the inflammatory react.
Posology,(quantities)
    Oral daily dosage, not more than 100 cc.per hour, Harmless on administration after healed, and could be used ashomecare in emergency of accidented.

发酵2年的多果汁液报告
——对伤口愈合研究课题的总结
药学系Dr.Chaiwat Chaiyasut博士与Miss Rosukon Poompanvong小姐
2005年
多果酶疗伤的内外应用
规定
水果
克
蜂蜜
水/cc.
Star Fruit杨桃
100
30
1,370
Guava番石榴
100
30
1,370
Tamarind  罗望子
100
30
1,370
Rambutan红毛丹
100
30
1,370
Longon龙眼
100
30
1,370
Mangos teen芒果的青少年
100
30
1,370
Banana香蕉
100
30
1,370
Sherry雪莉
100
30
1,370
Papaya木瓜
100
30
1,370
Pine Apple菠萝
100
30
1,370
Jack Fruit杰克果
100
30
1,370
……
密封的塑料瓶限使用1年,第二换新的塑料瓶并添加10%蜂蜜(脱水80%)塑料使用不应超过一年。第二年应检查pH值,电导率。

同义词
选择方言名称
描述
相关的植物原料,新鲜、皮(外观)
感官属性
温和的
芳香
气味新鲜道地
最初的甜、酸、口味(100%)
地理分布
是100%东南亚本地水果,亚洲主要是——在印度,中国南方,和西印度。非洲。
常规测试。
宏观检查、薄层色谱
亲脂性成分和菊苣a甲醇提取物的高效液相色谱法。
纯度检测，微生物
-多水果酵素中的沙门氏菌检验。
其他微-生物体限量–如下
准备好氧菌汤：不超过107／g；大肠杆菌不超过102／g，真菌–不超过105个/克
-内服：需氧菌不超过105个/克或毫升；
真菌–不超过104个/克或毫升；
-肠杆菌和一些革兰氏阴性菌不超过 103毫升/克或毫升；真菌–不超过102 / 克或毫升；
大肠杆菌O /克或毫升。
外用；
-需氧菌超过102／g或mL；……
农药残留量
按照国家规定确立。通常，果期的艾氏剂、狄氏剂的最大残留限量不超过0.05毫克/公斤
其他农药，WHO确定的药物质量控制方法，
植物和预测农药残留的膳食摄入量指南
重金属。
铅和镉在植物原料的最终剂型中的含量分别不超过10毫克和0.3毫克/公斤，
放射性残留
对锶90，碘131，铯铯—134，1237和钚239，参见WHO(世界卫生组织)对药用植物的质量控制方法的指南
其他纯度检测
化学试验和酸不溶性灰分，稀乙醛浸出试验，外来的有机物质，水分，灰分和水溶性提取物，–依照国家标准。
化学分析。
精油（0.08-0.32 %）；菊苣A（1.2～3.1 %）多水果酶的定量分析，菊苣A  ，异丁基酰胺和其他成分含量用高效液相色谱法
氨基酸和咖啡a衍生物用薄层色谱法和高效液相色谱定量分析。
主要化学成分
   已被检测出的化学物质，包括氨基酸，聚烯烃，polyalkynes，咖啡a衍生物和多糖
挥发油中，冰片，乙酸龙脑酯，十五烷
-8 -（大根香叶烯D）
，石竹烯和石竹烯环氧化物。
   直链脂肪酸与烯或炔……
   咖啡酸的酯衍生物的菊苣酸是主要的活性化合物……
菊苣酸的甲基酯和其他衍生物
多糖成分的液体是从水果两类：平均相对分子质量约3500（例如PS I）
   其他成分包括吡咯兹定生物碱微量（0.006%）。这些浓度的生物碱，被认为是无毒的。
具有代表性的成分结构如下。
剂型。
果实多液制剂有内部和外部使用。
药用的用途。以临床数据为准。
多果液口服治疗感冒和呼吸道和泌尿道感染。
治疗这些感染通常被认为是由免疫反应的刺激引起的。外部用途包括炎症性皮肤疾病，伤口愈合
   不支持使用民间医药的描述方式来描述实验或化学数据。
   其他医疗用途，包括酵母感染治疗，放射疗法的副作用，类风湿关节炎，血液中毒，食物中毒。
药理学。
实验药理学
   目前主张多果液有效性作为刺激免疫系统是基于大量的科学研究。免疫刺激作用的机理：吞噬和刺激成纤维细胞的激活；增加呼吸活性和白细胞的迁移率增加。对mutifruit液体提取物的标准化的吞噬活性已被确定。
多果液显着增加的吞噬人类粒细胞
百分比和在体外刺激酵母颗粒的吞噬
。也有报道，经多果液液治疗后。对肾上腺皮质的抑制活性，备解素（一种能消灭细菌和病毒的血清）产生的刺激（血清蛋白可中和病毒和细菌），和干扰素产生的刺激
多果液的药理活性证明是五个成分的属性，即生物碱，咖啡a衍生物，polyalkynes，聚烯烃，和多糖
水溶性酰胺，生物碱，和咖啡a衍生物看来是通过刺激中性粒细胞的吞噬作用，多果液的乙醇提取物通过刺激中性粒细胞的吞噬作用。
高分子多糖，包括heteroxylan，激活phacgocytosis，和阿拉伯半乳聚糖，从而促进肿瘤坏死因子的释放和白细胞介素1和β干扰素的生产，也与口服复合果液有关。乙醇提取物整体的免疫刺激活性，似乎依赖于几种成分的组合效应
多果液局部应用传统上被用来促进伤口愈合。有鉴于此，泰国罗勇健康农场的rosukon poompanvong博士，第一次发表论文表述，是他研究了多果液对链球菌局部和组织透明质酸酶的影响。
组织和细菌透明质酸酶的抑制被认为是防止病原体扩散到身体的其他部位。除了直接抗透明质酸酶的活性，对透明质酸酶系统的影响是间接的。
通过增加成纤维细胞活性的新的组织产生刺激，刺激血液和组织中产生的吞噬作用，似乎是参与这一机制。
多糖组分似乎形成了透明质酸–多糖复合物，间接地促进伤口愈合的透明质酸酶的抑制作用。
在体外实验中，乙醇提取物（65%）通过多果液体积发酵（2岁）抑制小鼠成纤维细胞胶原浓度（通过测量胶原晶格直径）。
小鼠巨噬细胞预处理与多糖是从多果液上清中分离发酵（2岁）增加生产的α，白细胞介素-1和肿瘤坏死情况，干扰素-β- 2的增加对肿瘤细胞的细胞毒性和微生物（杜氏enreitii
禁忌
外用–打开发酵2年的多果液瓶时，有可能爆炸和伤人，所以！必须去掉水平层上的复盖物。
无致敏性。
内用
不应该用于严重的疾病如艾滋病，HIV感染，多硬化胶原性疾病和自身免疫性疾病。
警告
一般注意事项
须持续使用约7周，每周一次直到恢复。
致癌，诱变，生育障碍。
与癌性试验结果均为阴性的致突变性。剂量为500毫克/毫升的多糖浓度引起姐妹染色单体交换或染色体结构畸变没有增加。
妊娠：致畸作用
未见可靠的研究。因此，不推荐妊娠期间用药。
儿科用的
推荐使用发酵两年的多果发酵液，用于刚出世的，直接滴服，小孩服用则按医嘱，也可作为外用治疗。
其他预防措施
没有关于药物相互作用的信息，药物和实验室试验相互作用，或非–畸形对妊娠结局的影响
偶尔，有一些炎症的愈合期及必须持续至症状逐渐好转和痊愈，不超过2-3天的炎症反应。
剂量
每日口服剂量，不超过100CC。每小时，无害化管理后愈合，可作为紧急事故的家庭护理。

从多水果液体发酵（2年）液中分离纯化的多糖在软琼脂中可增强人多形核白细胞的自发运动，增加这些细胞杀死金黄色葡萄球菌的能力。
被激活后的人单核细胞，分泌肿瘤坏死因子-α，白细胞介素1，和白细胞介素6，而11种人白细胞抗原表达不受影响。
纯化后的咖啡酸衍生物被证明具有抗病毒活性。
水疱性口炎病毒（VSV）与125μ克/毫升的菊苣酸一起4小时就可减少超过50%的小鼠L-929小鼠细胞中的病毒粒子的数量。
最近在健康农场进行的6个对照临床综合系统的试验，四个试验研究证明化疗毒副反应减少，三个对特异性免疫参数有调节作用。
评价认为，多果汁液体发酵（2年）液是有效的免疫调节剂。
大规模的纵向试验（500例患者）研究了含有冷冻干产物软膏
的影响。软膏用于治疗皮肤炎症，创伤，烧伤，湿疹，疱疹，腿部溃疡和静脉曲张。
临床治疗观察到软膏的疗效达85.5%。治疗时间7～15天。

qljr520

翻译成中文，我也看不懂了，郁闷

阳光899

太专业了，我也看得不明白

陈香酵素

感恩，收藏起，慢慢看

富晶

{:soso_e179:}

zap

辛苦了，谢谢。

印印

感恩分享

cindyma

谢谢！辛苦了。能有人解释就更好了{:soso_e100:}

以玉交友

{:soso_e183:}

淡泊

看着不是很明白，还是先收藏吧。一定用的着。